HSP60 Antibody / HSPD1

TLR2

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Immunocytochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P63039

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of rat HSP60 (YDAMLGDFVNMVEK) was used as the immunogen for this HSP60 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

The lyophilized HSP60 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

Limitations

This HSP60 antibody is available for research use only.

Storage Conditions

The lyophilized HSP60 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Variations in secondary/substrate sensitivities and test protocols may require the HSP60 antibody to be titrated for optimal performance.

Location

Cytoplasm (mitochondria)

Image Legend

Immunofluorescent staining of FFPE human A431 cells with HSP60 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.