Myeloperoxidase Antibody / MPO

Myeloperoxidase is a mammalian phagocyte hemoprotein though to primarily mediate host defense reactions. It is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL

UniProt

P05164

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the C-terminus of the human protein (KNNIFMSNSYPRDFV) was used as the immunogen for this Myeloperoxidase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

The lyophilized Myeloperoxidase antibody can be stored at 4oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Myeloperoxidase antibody is available for research use only.

Storage Conditions

The lyophilized Myeloperoxidase antibody can be stored at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Titration of the pAb may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear and cytoplasmic

Image Legend

IHC staining of FFPE mouse spleen tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.