Lamin B1 Antibody / LMNB1

Lamin B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. They are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

UniProt

P20700

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 570-586 (FHQQGTPRASNRSCAIM-human) were used as the immunogen for this Lamin B1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, IP

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Lamin B1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Lamin B1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Lamin B1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Lamin B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear membrane, nuclear

Image Legend

IHC staining of FFPE human lung cancer tissue with Lamin B1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.