GST pi Antibody / GSTP1

Glutathione S-transferases pi, also known as GST pi, GSTP1 and GST3, is present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. It is mapped to chromosome 11 . Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GST pi does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P09211

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids PEYVNLPINGNGKQ were used as the immunogen for this GST pi antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the GST pi antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GST pi antibody is available for research use only.

Storage Conditions

After reconstitution, the GST pi antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the GST pi antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human ovarian cancer tissue with GST pi antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.