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BDNF Antibody

Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P23560

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

An amino acid sequence from the N-terminal of human BDNF (HSDPARRGELSVCDSISEWV) was used as the immunogen for this BDNF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the BDNF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This BDNF antibody is available for research use only.

Storage Conditions

After reconstitution, the BDNF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the BDNF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of BDNF antibody and Lane 1: rat brain; 2: mouse brain; 3: MCF-7; Predicted size: 28KD; Observed size: 37KD

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