IL3 Antibody

Interleukin 3 is a glycoprotein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (rat protein tested) ; request BSA free format for coating

Host

Rabbit

Reactivity

Rat

Immunogen

Rat partial recombinant protein (AA 28-166) was used as the immunogen for this IL3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA (protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the IL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This IL3 antibody is available for research use only.

Storage Conditions

After reconstitution, the IL3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the IL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.