Claudin 5 Antibody

Claudin-5, also known as BEC1, is a protein that in humans is encoded by the CLDN5 gene. By use of yeast artificial chromosomes, Claudin-5 is localized between polymorphic markers D22S944 and D22S941 on chromosome 22q11, both of which are deleted in more than 80% of VCFS patients. This gene encodes a member of the claudin family. Family members are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Additionally, it plays a major role in tight junction-specific obliteration of the intercellular space.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Amino acids 191-218 (RPDLSFPVKYSAPRRPTATGDYDKKNYV-human) were used as the immunogen for the Claudin-5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Claudin 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Claudin 5 antibody is available for research use only.

Storage Conditions

After reconstitution, the Claudin 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Claudin 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.