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NGF Antibody / Beta Nerve Growth Factor

Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. The nucleotide sequence of human and mouse beta-NGF are very similar. The beta-subunits (NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested)

UniProt

P01138

Host

Rabbit

Reactivity

Human

Immunogen

Human partial recombinant protein (AA 122-241) was used as the immunogen for this NGF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA (protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 0.025% sodium azide

Reconstitution

After reconstitution, the NGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NGF antibody is available for research use only.

Storage Conditions

After reconstitution, the NGF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the NGF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of NGF antibody and Lane 1: Recombinant human protein 10ng; 2: 5ng; 3: 2.5ng

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