Mouse TNF alpha Antibody

Tumor Necrosis Factor alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It binds and functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation and has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot (recombinant protein) : 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (mouse protein tested)

UniProt

P06804

Host

Rabbit

Reactivity

Mouse

Immunogen

Mouse partial recombinant protein (AA 80-235) was used as the immunogen for this mouse TNF alpha antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB (recombinant protein), IHC-P, ELISA (recombinant protein)

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 0.025% sodium azide

Reconstitution

After reconstitution, the mouse TNF alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This mouse TNF alpha antibody is available for research use only.

Storage Conditions

After reconstitution, the mouse TNF alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the mouse TNF alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.