Beta Actin Antibody

The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of the protein that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids DDDIAALVIDNGSGK were used as the immunogen for this beta-Actin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

AC-15

Applications

WB, IHC-P

Purity

Unpurified ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the beta-Actin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This beta-Actin antibody is available for research use only.

Storage Conditions

After reconstitution, the beta-Actin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting points. Titration of the beta-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membranous

Image Legend

Western blot testing of beta-Actin antibody and rat tissue: ovary (1), testis (2), heart (3), brain (4) ; and human cell lines, lanes 5: A453; 6: HeLa; 7: SMMC-7721; 8: HT1080; 9: SW620 cell lysate