Alpha Tubulin Antibody / TUBA1A

Alpha-tubulin (b-alpha-1) mRNA is expressed at a molecular weight of about 50-55,000D. The 3-prime UTR of the protein is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene IL-alpha-T1. Alpha tubulin is a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P68366

Host

Mouse

Reactivity

Human, Mouse, Rat, Chicken

Immunogen

Microtubules from chicken embryo brain was used as the immunogen for this Alpha Tubulin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

DM1A

Applications

WB, IHC-P

Purity

Unpurified ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Alpha Tubulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Alpha Tubulin antibody is available for research use only.

Storage Conditions

After reconstitution, the Alpha Tubulin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Alpha Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) human SH-SY5Y, 2) human Jurkat, 3) human A549, 4) rat brain, 5) mouse brain and 6) mouse thymus tissue lysate with Alpha Tubulin antibody. Predicted molecular weight ~50 kDa.