What is the catalog number for Alpha-Adaptin Antibody / AP2A1?

The catalog number for Alpha-Adaptin Antibody / AP2A1 is R30093.

What sizes are available for Alpha-Adaptin Antibody / AP2A1?

Alpha-Adaptin Antibody / AP2A1 is available in the following size(s): 100 µg.

Is Alpha-Adaptin Antibody / AP2A1 in stock?

Alpha-Adaptin Antibody / AP2A1 is currently made to order / not held in immediate stock. Contact us for lead time.

Alpha-Adaptin Antibody / AP2A1

Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC) and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL

UniProt

O95782

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

AP-2 adaptor polypeptides from bovine brain was used as the immunogen for this Alpha-Adaptin antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

100/2

Applications

WB

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the alpha-Adaptin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Alpha-Adaptin antibody is available for research use only.

Storage Conditions

After reconstitution, the Alpha-Adaptin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Alpha-Adaptin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) human SH-SY5Y, 2) human U-87 MG, 3) human MCF7, 4) human 293T, 5) rat brain, 6) rat C6 and 7) mouse brain tissue lysate with Alpha-Adaptin antibody. Predicted molecular weight ~108 kDa.