Alpha-Actinin Antibody

Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1.5-3 µg/mL, IHC (Frozen) : 1.5-3 µg/mL to detect in formalin or acetone fixed tissues

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit

Immunogen

Rabbit skeletal alpha-actinin was used as the immunogen for this alpha-Actinin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

EA-53

Applications

WB, IHC-P, IHC-F

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the alpha-Actinin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This alpha-Actinin antibody is available for research use only.

Storage Conditions

After reconstitution, the alpha-Actinin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the alpha-Actinin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) mouse heart, 4) mouse skeletal muscle and 5) human placenta lysate with alpha-Actinin antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.