Vinculin Antibody

Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and the two proteins exhibit a high level of sequence identity (greater than 95%) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, IHC (Frozen) : 2-4 µg/mL for formalin or acetone fixed tissues, ICC/IF: 2-4 µg/mL

Host

Mouse

Reactivity

Human, Mouse, Rat, Chicken

Immunogen

Human vinculin purified from uterus tissue was used as the immunogen for this Vinculin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

HVIN-1

Applications

WB, IHC-F, ICC/IF

Purity

Unpurified ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Vinculin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Vinculin antibody is available for research use only.

Storage Conditions

After reconstitution, the Vinculin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) human HeLa, 2) human HepG2, 3) monkey COS-7, 4) human U-87 MG, 5) rat PC-12, 6) rat RH35, 7) mouse HEPA1-6, and 8) mouse NIH3T3 lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.