Vimentin Antibody

The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. The Vimentin gene expresses in human lymphocytes and in Burkitts lymphoma cells and it is secreted by activated macrophages. The gene encoding human protein is located on the short arm of chromosome 10.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

Host

Mouse

Reactivity

Human, Rabbit, Pig, Rat

Immunogen

Pig eye lens vimentin was used as the immunogen for this Vimentin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

Applications

WB, IHC-P, IF

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Vimentin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Vimentin antibody is available for research use only.

Storage Conditions

After reconstitution, the Vimentin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear, cell membrane

Image Legend

Immunofluorescent staining of FFPE human intestinal cancer with Vimentin antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA for 20 min.