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Tenascin Antibody

The tenascins are a family of extracellular matrix proteins (ECMs) . Tenascin is an extracellular matrix protein. It expressed in an unusually restricted pattern during embryogenesis and has been implicated in a variety of morphogenetic phenomena. Human hexabrachion gene (tenascin) is mapped to chromosome 9, bands q32-q34. The coding region of the hexabrachion gene spans approximately 80 kilobases of DNA and consists of 27 exons separated by 26 introns.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, IHC (FFPE) : 2-4 µg/mL, IHC (Frozen) : 2-4 µg/mL to detect in formalin or acetone fixed tissues, Immunocytochemistry: Suitable

Host

Mouse

Reactivity

Human

Immunogen

Human tenascin was used as the immunogen for this Tenascin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

BC-24

Applications

WB, IHC-P, IHC-F, ICC

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Tenascin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Tenascin antibody is available for research use only.

Storage Conditions

After reconstitution, the Tenascin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Tenascin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC-P: Tenascin antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer, HRP-staining.

Documents

Product Datasheet

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