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Tau Antibody / MAPT

The microtubule-associated proteins (MAPs) coassemble with tubulin into microtubules in vitro. Microtubule-associated protein Tau appears to be enriched in axons. Tau is composed of 352 to 441 amino acids and migrate at 45-65KD. The isoforms differ from each other by the presence or absence of 29-amino acid or 58-amino acid inserts located in the N terminus and a 31-amino repeat located in the C terminus. Tau is important in establishing and maintaining neuronal morphology and is a major component of the neurofibrillary tangles (NFTs) characteristic of Alzheimers brain.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

Human, Bovine

Immunogen

Bovine microtubule-associated proteins were used as the immunogen for this Tau antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

TAU-2

Applications

WB, IHC-P

Purity

Unpurified ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Tau antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Tau antibody is available for research use only.

Storage Conditions

After reconstitution, the Tau antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Tau antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Tau antibody and Lane 1: rat brain (40ug) ; 2: rat brain (50ug) ; 3: mouse brain (40ug) ; 4: mouse brain (50ug) . Expected molecular weight: 50-80 kDa.

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