Synaptophysin Antibody

Synaptophysin (SYP) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Ozcelik et al. (1990) concluded that the gene has 7 exons distributed over about 20 kb. The SYP locus mapped to Xp11.23-p11.22. Regionalization of the gene on the X chromosome was also done with hamster/human hybrid cells in which various portions of the human X chromosome were present.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IHC (Frozen) : 1-2 µg/mL, formalin or acetone fixed tissues

Host

Mouse

Reactivity

Human, Rat

Immunogen

Rat retina synaptosome was used as the immunogen for this Synaptophysin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

SVP-38

Applications

WB, IHC-P, IHC-F

Purity

Unpurified ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Synaptophysin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Synaptophysin antibody is available for research use only.

Storage Conditions

After reconstitution, the Synaptophysin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Synaptophysin antibody may be required due to differences in protocols and secondary/substrate sensitivity.