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PSTAIR Antibody

The cyclin-dependent protein kinases (CDKs) regulate major cell cycle transitions in eukaryotic cells. CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIRE motif found in prototypic CDC2 kinases. CDC2L1 is referred as PITSLRE B, based on the amino acid sequence of the region corresponding to the conserved CDC2 PSTAIRE box.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Synthetic 16 amino acid oligopeptide containing the PSTAIR sequence was used as the immunogen for this PSTAIR antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

PSTAIR

Applications

WB

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the PSTAIR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PSTAIR antibody is available for research use only.

Storage Conditions

After reconstitution, the PSTAIR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the PSTAIR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) human A549, 2) human 293T, 3) human Caco-2, 4) human ThP-1, 5) rat C6, 6) rat PC-12, 7) mouse RAW264.7 and 8) mouse NIH 3T3 cell lysate with PSTAIR antibody. Expected molecular weight in CDK1: ~34 kDa.

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