Neurofilament Antibody (Light)

Neurofilaments are composed of three neuron-specific proteins with apparent molecular masses of 68kD (NFL), 125kD (NFM) and 200kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. NF68, also called Neurofilament Protein Light (NFL) is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, IHC (FFPE) : 2-4 µg/mL, IHC (Frozen) : 2-4 µg/mL; formalin or acetone fixed tissues

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Pig spinal cord was used as the immunogen for this light Neurofilament antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

NR4

Applications

WB, IHC-P, IHC-F

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Neurofilament antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Neurofilament antibody is available for research use only.

Storage Conditions

After reconstitution, the Neurofilament antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Neurofilament antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membranous

Image Legend

IHC staining of FFPE mouse brain tissue with Light Chain Neurofilament antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.