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Myoglobin Antibody

Myoglobin (MB) also known as PVALB, is asingle-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. Two myoglobin variants were found. The locus mapped to 22q11-22q13. The myoglobin gene is about 10.5 kb long and contains two introns as in the case with hemoglobin genes. Myoglobin may serve a variety of functions in muscular oxygen supply, such as O2 storage, facilitated O2 diffusion, and myoglobin-mediated oxidative phosphorylation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 2-4 µg/mL, IHC (P) : 2-4 µg/mL

Host

Mouse

Reactivity

Human, Rat

Immunogen

Purified human skeletal muscle myoglobin was used as the immunogen for this Myoglobin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

MG-1

Applications

WB, IHC-P

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

The lyophilized Myoglobin antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Myoglobin antibody is available for research use only.

Storage Conditions

The lyophilized Myoglobin antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Myoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of Myoglobin antibody and rat skeletal muscle tissue lysate. Predicted molecular weight ~17 kDa.

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