MAP2 Antibody / Microtubule-associated protein 2

MAP2, a 280-kD protein, is highly concentrated in neuronal somata and dendrites. Microtubule-associated protein 2 (MAP2) is a neurosteroid receptor. MAP2 gene contains 19 exons, and located in segment 2q34-q35. The transgenic MAP2c was present in dendrites but not in axons but transgenic MAP2c messenger RNA was limited to cell bodies.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

Human, Rat, Mouse

Immunogen

Rat brain microtubule-associated proteins were used as the immunogen for this MAP2 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

HM-2

Applications

WB, IHC-P

Purity

Unpurified ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the MAP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MAP2 antibody is available for research use only.

Storage Conditions

After reconstitution, the MAP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting points. Titration of the MAP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC-P testing of rat brain tissue with MAP2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.