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MAP1 Antibody

Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins (MAPs) . MAP1 is one of the major neuronal MAPs as well as being the largest (350KD) . MAPs include MAP1A, MAP1B, and MAP2. MAP1a is a single-copy gene spanning 10.5 kb. MAP1a coding sequence is contained in five exons. MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-2 µg/mL, IHC (FFPE) : 1-2 µg/mL, IHC (Frozen) : 1-2 µg/mL--formalin or acetone fixed tissues

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Rat brain microtubule-associated proteins were used as the immunogen for this MAP1 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

HM-1

Applications

WB, IHC-P, IHC-F

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the MAP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MAP1 antibody is available for research use only.

Storage Conditions

After reconstitution, the MAP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC-P: MAP1 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer, HRP-staining.

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