What is the catalog number for MAP Kinase Antibody, Activated (Diphosphorylated ERK-1&2)?

The catalog number for MAP Kinase Antibody, Activated (Diphosphorylated ERK-1&2) is R30048.

What sizes are available for MAP Kinase Antibody, Activated (Diphosphorylated ERK-1&2)?

MAP Kinase Antibody, Activated (Diphosphorylated ERK-1&2) is available in the following size(s): 100 µg.

MAP Kinase Antibody, Activated (Diphosphorylated ERK-1&2)

In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein (MAP) kinase pathways. The central core of each MAP kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: Suitable

Host

Mouse

Reactivity

Human, Mouse, Rat, yeast

Immunogen

A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH was used as the immunogen for this MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) .

Clonality

Monoclonal

Isotype

IgG1

Clone

MAPK-YT

Applications

WB, IHC-P, ICC

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) is available for research use only.

Storage Conditions

After reconstitution, the MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) may be required due to differences in protocols and secondary/substrate sensitivity.