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Glucagon Antibody

Glucagon is a member of a multigene family that includes secretin. Mature, it is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. The human gene is approximately 9.4 kb long and contains 6 exons and 5 introns, and assigned to 2q36-2q37.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 5 µg/mL

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit

Immunogen

Polymerized porcine glucagon was used as the immunogen for this Glucagon antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

K79bB10

Applications

IHC-P, IF

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Glucagon antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Glucagon antibody is available for research use only.

Storage Conditions

After reconstitution, the Glucagon antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Glucagon antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of FFPE mouse pancreas tissue with Glucagon antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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