GFAP Antibody
Glial fibrillary acidic protein is an intermediate filament protein of 52kDa. Its gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in the protein are associated with Alexander disease.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.4-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL (formalin or acetone fixed tissues), Immunofluorescence: 2-4 µg/mL
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Glial fibrillary acidic protein from pig spinal cord was used as the immunogen for this GFAP antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
G-A-5
Applications
WB, IHC-P, IHC-F, IF
Purity
Unpurified ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the GFAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This GFAP antibody is available for research use only.
Storage Conditions
After reconstitution, the GFAP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Applications Notes
The stated application concentrations are suggested starting points. Titration of the GFAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of FFPE rat brain tissue with GFAP antibody (green), MBP antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.
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