Gelsolin Antibody

Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IHC (Frozen) : 1-2 µg/mL for acetone fixed tissues

Host

Mouse

Reactivity

Human, Rabbit

Immunogen

Human plasma gelsolin was used as the immunogen for this Gelsolin antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

GS-2C4

Applications

WB, IHC-P, IHC-F

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Gelsolin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Gelsolin antibody is available for research use only.

Storage Conditions

After reconstitution, the Gelsolin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Gelsolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.