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Cyclin A Antibody

The mammalian A-type cyclin family consists of 2 members, Cyclin A1 (CCNA1) and Cyclin A2 (CCNA2) . Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunocytochemistry: 1 µg/mL

Host

Mouse

Reactivity

Human, Mouse, Bovine

Immunogen

Recombinant bovine cyclin A was used as the immunogen for this Cyclin A antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

CY-A1

Applications

WB, ICC

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Cyclin A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cyclin A antibody is available for research use only.

Storage Conditions

After reconstitution, the Cyclin A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Cyclin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Cyclin A antibody and Lane 1: HeLa; 2: Jurkat cell lysate. Predicted molecular weight: 50-55 kDa.

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