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COL3A1 Antibody / Collagen III

Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, IHC (Frozen) : 0.5-1 µg/mL-formalin/acetone fixed tissues

Host

Mouse

Reactivity

Human, Rat

Immunogen

Human collagen type III was used as the immunogen for this COL3A1 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

Col-29

Applications

WB, IHC-F

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the COL3A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This COL3A1 antibody is available for research use only.

Storage Conditions

After reconstitution, the COL3A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the COL3A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of COL3A1 antibody and Lane 1: HT1080; 2: COLO320 cell lysate. Expected molecular weight ~139 kDa, observed here at ~70 kDa.

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