Cytokeratin 18 Antibody
Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the Cytokeratin 18/Keratin 18 protein is coded for by 7 exons. By Southern blotting using the genomic DNA PCR product, the gene for Cytokeratin 18 has been assigned to chromosome 12. Mutation of human Keratin 18 has been associated with cryptogenic cirrhosis.
Product Specifications
CAS Number
9007-83-4
Specifications
Host
Mouse
Reactivity
Human
Immunogen
The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines was used as the immunogen for this Cytokeratin 18 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
CY-90
Applications
WB, IHC-P, IHC-F, IF
Purity
Unpurified ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
Limitations
This Cytokeratin 18 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
Image Legend
Immunofluorescent staining of FFPE human rectal cancer tissue with Cytokeratin 18 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
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