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CEA Antibody / CEACAM5

Carcinoembryonic antigen is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

Carcinoembryonic antigen isolated from a human colon adenocarcinoma cell line was used as the immunogen for this CEA antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

C6G9

Applications

WB, IHC-P

Purity

Ascites

Format

Ascites

Buffer

Lyophilized from mouse ascites fluid with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the CEA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CEA antibody is available for research use only.

Storage Conditions

The CEA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the CEA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of CEA antibody and Lane 1: Recombinant human CEA protein 10ng; Lane 2: 5ng; 3: 2.5ng; 4: 1.25ng

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