Cathepsin L Antibody

Cathepsin L is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. Cathepsin L has been implicated in pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Human liver cathepsin L consists of a heavy chain of about 25 kD and a light chain of about 5 kD. The gene is mapped to 9q21-q22. Cathepsin L is required for endothelial progenitor cell-induced neovascularization.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 4 µg/mL-formalin/acetone fixed tissues

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Procathepsin L isolated from the human lung cancer cell line EPLC 32M1 was used as the immunogen for this Cathepsin L antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

33/2

Applications

WB, IHC-F

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

After reconstitution, the Cathepsin L antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cathepsin L antibody is available for research use only.

Storage Conditions

After reconstitution, the Cathepsin L antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Cathepsin L antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human A549 cell lysate with Cathepsin L antibody. Expected molecular weight: 38-41 kDa with multiple smaller processed/active forms.