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Calponin 2 Antibody

Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: Suitable

Host

Mouse

Reactivity

Human, Chicken, Turkey

Immunogen

Turkey gizzard calponin was used as the immunogen for this Calponin 2 antibody

Clonality

Monoclonal

Isotype

IgG1

Clone

CP-19

Applications

WB, IHC-P, ICC

Purity

Ascites

Format

Ascites

Buffer

0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

Reconstitution

The lyophilized Calponin 2 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

Limitations

This Calponin 2 antibody is available for research use only.

Storage Conditions

The lyophilized Calponin 2 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

Formulation

0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

Applications Notes

Variations in secondary/substrate sensitivities and test protocols may require the Calponin 2 antibody to be titrated for optimal performance.

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