Recombinant PLAP Antibody / Placental Alkaline Phosphatase / N-Terminal

Alkaline phosphatase, placental type is a membrane-bound glycosylated dimeric enzyme, also referred to as the heat-stable form, that is expressed primarily in the placenta, although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. <br><br>PLAP is a tumor marker, especially in seminoma and ovarian cancer. PLAP is reliable only in non-smokers, as smoking interferes with measurement of PLAP, since serum concentrations of PLAP are increased up to 10-fold in smokers and its measurement is therefore of little value in this group. [Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:500-1:1000, Western Blot: 1:1000-1:2000

UniProt

P05187

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the N-terminus of human placental alkaline phosphatase was used as the immunogen for the recombinant PLAP antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM317

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant PLAP antibody at -20oC.

Limitations

This recombinant PLAP antibody is available for research use only.

Storage Conditions

Store the recombinant PLAP antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant PLAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, cell membrane

Image Legend

Western blot testing of human A431 cell lysate with recombinant PLAP antibody at 1:2000 dilution. Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.