Recombinant NeuN Antibody / Fox3 / N-Terminal

RNA binding protein fox-1 homolog 3 (RBFOX3), also called Neuronal nuclei antigen and Fox-1 homolog C, is a pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD) . [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:500-1:1000, Western Blot: 1:1000-1:2000

UniProt

A6NFN3

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A peptide corresponding to the N-terminus of human NeuN/RBFOX3 was used as the immunogen for the recombinant NeuN antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM312

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant NeuN antibody at -20oC.

Limitations

This recombinant NeuN antibody is available for research use only.

Storage Conditions

Store the recombinant NeuN antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant NeuN antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

Western blot testing of human brain lysate with recombinant NeuN antibody at 1:1000 dilution. Expected molecular weight: 46-48 kDa (two isoforms may be visualized) .