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Recombinant BRAF Antibody / / N-Terminal Region

This antibody reacts to human serine/threonine-protein kinase B-raf (BRAF) . It may also react to mouse B-raf, as predicted by immunogen homology.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:200-1:500, Western Blot: 1:100-1:200

UniProt

P15056

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the residues near N-terminus of human B-raf was used as the immunogen for the recombinant BRAF antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM308

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant BRAF antibody at -20oC.

Limitations

This recombinant BRAF antibody is available for research use only.

Storage Conditions

Store the recombinant BRAF antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Location

Cytoplasmic

Image Legend

Western blot testing of human K562 cell lysate with recombinant BRAF antibody at 1:100 dilution. Predicted molecular weiight ~84 kDa.

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