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Recombinant p27 Antibody / C-Terminal

This antibody reacts to human p27Kip1 (Cyclin-dependent kinase inhibitor 1B) . It may also react to mouse p27Kip1, as predicted by immunogen homology.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:100-1:200, Western Blot: 1:500-1:1000

UniProt

P46527

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the C-terminus of human p27Kip1 was used as the immunogen for the recombinant p27 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM302

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant p27 antibody at -20oC.

Limitations

This recombinant p27 antibody is available for research use only.

Storage Conditions

Store the recombinant p27 antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant p27 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Location

Nuclear

Image Legend

IHC testing of formalin fixed and paraffin embedded human breast cancer tissue with recombinant p27 antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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