Recombinant phospho-EIF2A Antibody (Ser51)

This antibody reacts to human eIF-2a (Eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. There is no cross-reactivity to eIF-2a without phosphorylation at Ser51.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:100-1:200, Western Blot: 1:100-1:200

UniProt

P05198

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding the the amino acids surrounding phosphorylated serine 51 was used as the immunogen for the recombinant phospho-EIF2A antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM298

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant phospho-EIF2A antibody at -20oC.

Limitations

This recombinant phospho-EIF2A antibody is available for research use only.

Storage Conditions

Store the recombinant phospho-EIF2A antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-EIF2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat

Location

Cytoplasmic

Image Legend

Western blot testing of lysate from human HeLa cells, untreated (-) or treated (+) with Calyculin A (CalA), with recombinant phospho-EIF2A antibody at 1:200 dilution. Predicted molecular weight ~36 kDa.