Recombinant LC3B Antibody / N-Terminal

This antibody reacts to human LC3B (Autophagy-related protein LC3 B; Microtubule-associated proteins 1A/1B light chain 3B) . It may also react to mouse or rat LC3B, as predicted by immunogen homology.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:100-1:200, Immunocytochemistry: 1:100-1:200, Western Blot: 1:100-1:200

UniProt

Q9GZQ8

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the N-terminus of human LC3B was used as the immunogen for the recombinant LC3B antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM293

Type

Recombinant

Applications

WB, IHC, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant LC3B antibody at -20oC.

Limitations

This recombinant LC3B antibody is available for research use only.

Storage Conditions

Store the recombinant LC3B antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

ICC staining of human HeLa cells untreated or treated with chloroquine (CHQ), with recombinant LC3B antibody (red) at 1:200 dilution. Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue) .