Recombinant BRAF V600E Antibody

This antibody reacts to the BRAF V600E mutant. No cross reactivity seen with wild type BRAF.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-5 µg/mL, Immunocytochemistry: 2-10 µg/mL, Western Blot: 0.5-2 µg/mL

UniProt

P15056

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to BRAF V600E mutant was used as the immunogen for the recombinant BRAF V600E antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM8

Type

Recombinant

Applications

WB, IHC, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant BRAF V600E antibody at -20oC.

Limitations

This recombinant BRAF V600E antibody is available for research use only.

Storage Conditions

Store the recombinant BRAF V600E antibody at -20°C.

Formulation

1 mg/mL in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

Western blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein with recombinant BRAF V600E antibody.