Recombinant HA Tag Chimeric Human Antibody

This antibody reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. An anti-human IgG secondary is required for this mAb. No cross reactivity with any endogenous protein.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (Capture) : 0. 5 µg/mL-2 µg/mL, ELISA (Detect) : 0.01 µg/mL-0.5 µg/mL, Western Blot: 0.1 µg/mL-1 µg/mL

Host

Rabbit

Immunogen

A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106) was used as the immunogen for the recombinant HA Tag Chimeric Human antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RMH02

Type

Recombinant

Applications

ELISA (Capture or Detect), WB

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant HA Tag Chimeric Human antibody at -20oC.

Limitations

This recombinant HA Tag Chimeric Human antibody is available for research use only.

Storage Conditions

Store the recombinant HA Tag Chimeric Human antibody at -20°C.

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant HA Tag Chimeric Human antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using recombinant HA Tag Chimeric Human antibody at 0.1 ug/ml, followed by a HRP conjugated anti-Human IgG secondary.