Recombinant HA Tag Antibody

This antibody reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein.

Product Specifications

CAS Number

9000-83-3

Specifications

Western Blot: 0.1 µg/mL-1 µg/mL, Immunoprecipitation: 0.5 µg/mL-2 µg/mL, Immunocytochemistry: 0.1 µg/mL-1 µg/mL, Immunohistochemistry: 0.1 µg/mL-0.5 µg/mL

Host

Rabbit

Immunogen

A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106) was used as the immunogen for the recombinant HA Tag antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM305

Type

Recombinant

Applications

WB, IP, ICC, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant HA Tag antibody at -20oC.

Limitations

This recombinant HA Tag antibody is available for research use only.

Storage Conditions

Store the recombinant HA Tag antibody at -20°C.

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant HA Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of recombinant HA Tag antibody (0.1ug/ml) with lysate from HEK293 cells transfected (+) or untransfected (-) with an HA-tag KRas DNA construct.