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Recombinant Neurofilament Antibody (light chain)

This recombinant Neurofilament antibody (light chain) reacts to human Neurofilament-L (NF-L) .

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1:1000-1:2500 (1), Western blot: 1:1000-1:2500

UniProt

P07196

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the C-terminus of human Neurofilament-L (NF-L) was used as the immunogen for this recombinant Neurofilament antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM280

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Neurofilament antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Neurofilament antibody is available for research use only.

Storage Conditions

Store the recombinant Neurofilament antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Product Datasheet

https://www.nsjbio.com/tds-pdf/recombinant-neurofilament-antibody-light-chain-rm280-r20297

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Neurofilament antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

CAS Number

9000-83-3

Image Legend

Western blot of human brain lysate using recombinant Neurofilament antibody at 1:2500. Predicted molecular weight: 62-68 kDa.

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