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Recombinant Cytokeratin 18 Antibody

This recombinant Cytokeratin 18 antibody reacts to human CK18/Cytokeratin-18.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:1000-1:4000 (1), Western blot: 1:1000-1:2000

UniProt

P05783

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the N-terminus of human Cytokeratin 18 was used as the immunogen for this recombinant Cytokeratin 18 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM279

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Cytokeratin 18 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Cytokeratin 18 antibody is available for research use only.

Storage Conditions

Store the recombinant Cytokeratin 18 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Location

Cytoplasmic

Image Legend

Western blot of human MCF7 and HeLa cell lysate using recombinant Cytokeratin 18 antibody at 1:1000. Predicted molecular weight ~48 kDa.

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