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Recombinant p65 Antibody

This recombinant p65 antibody reacts to human transcription factor p65 (NFkB p65 subunit) .

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:500-1:1500 (1), Western blot: 1:500-1:1000

UniProt

Q04206

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the area near the C-terminus of human transcription factor p65 (NFkB p65 subunit) was used as the immunogen for this recombinant p65 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM273

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant p65 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant p65 antibody is available for research use only.

Storage Conditions

Store the recombinant p65 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human breast cancer tissue with recombinant p65 antibody at 1:1250.

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