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Recombinant Smac Antibody

This recombinant Smac antibody reacts to human Smac (Diablo homolog, mitochondrial) .

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:500-1:1000 (1), Western blot: 1:1000-1:2000

UniProt

Q9NR28

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the C-terminus of human Smac/Diablo was used as the immunogen for this recombinant Smac antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM271

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Smac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant Smac antibody is available for research use only.

Storage Conditions

Store the recombinant Smac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Smac antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Location

Cytoplasmic

Image Legend

Western blot of human HeLa and MCF7 cell lysate using recombinant Smac antibody at 1:1000. Predicted molecular weight: 21~27 kDa.

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