Recombinant ITGA4 Antibody

This recombinant ITGA4 antibody reacts to the cytoplasmic domain of human Integrin alpha 4.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:1000-1:5000 (1), Western blot: 1:1000-1:2000

UniProt

P13612

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the C-terminus of human Integrin alpha 4 was used as the immunogen for this recombinant ITGA4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM268

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant ITGA4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant ITGA4 antibody is available for research use only.

Storage Conditions

Store the recombinant ITGA4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant ITGA4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Location

Cytoplasmic, membranous

Image Legend

Western blot testing of human Jurkat cell lysate with recombinant ITGA4 antibody at 1:1000. Predicted molecular weight ~115 kDa, routinely observed at ~150 kDa.