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Recombinant phospho-Stat3 Antibody (Tyr705)

This recombinant phospho-Stat3 antibody reacts to human Stat3 only when phosphorylated at Tyr705. There is no cross-reactivity to Stat3 without phosphorylation at Tyr705. It may also react to mouse or rat phospho-Stat3 (Tyr705), as predicted by immunogen homology.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:1000-1:10;000 (1), Western blot: 1:1000-1:2000

UniProt

P40763

Host

Rabbit

Reactivity

Human

Immunogen

A phospho-peptide corresponding to human phospho-Stat3 (Tyr705) was used as the immunogen for this recombinant phospho-Stat3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM261

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant phospho-Stat3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant phospho-Stat3 antibody is available for research use only.

Storage Conditions

Store the recombinant phospho-Stat3 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Stat3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Prediction Reactivity

Mouse, Rat

Image Legend

Western blot of A431 cell lysate, untreated (-) or treated (+) with EGF, using recombinant phospho-Stat3 antibody at 1:1000.

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