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Recombinant ACTC1 Antibody

The recombinant ACTC1 antibody reacts to human and mouse alpha cardiac actin (Actin, alpha cardiac muscle 1) . It may also react to bovine or rat alpha-cardiac actin, as predicted by immunogen homology.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:1000-1:2000 (1), Western blot: 1:1000-1:2000

UniProt

P68032

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A peptide corresponding to the N-terminus of human alpha-cardiac Actin was used as the immunogen for this recombinant ACTC1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM257

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant ACTC1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant ACTC1 antibody is available for research use only.

Storage Conditions

Store the recombinant ACTC1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant ACTC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Prediction Reactivity

Bovine, Rat

Image Legend

Western blot testing of mouse heart lysate with recombinant ACTC1 antibody at 1:1000. Predicted molecular weight ~42 kDa.

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