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Recombinant AKT1 Antibody

This recombinant AKT1 antibody may also react to bovine, mouse or rat AKT1, as predicted by immunogen homology

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:500-1:1000 (1), Western blot: 1:1000-1:2000

UniProt

P31749

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the C-terminus of human Akt1 was used as the immunogen for this recombinant AKT1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM252

Type

Recombinant

Applications

WB, IHC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant AKT1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Limitations

This recombinant AKT1 antibody is available for research use only.

Storage Conditions

Store the recombinant AKT1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Prediction Reactivity

Bovine, Mouse, Rat

Image Legend

Western blot testing of 293 cell lysate with recombinant AKT1 antibody at 1:1000. Predicted molecular weight ~56 kDa.

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